• Enhanced Formula: We’ve improved our ALLROUNDER Balancer formula, adding Pro’Age to support healthier pet ageing.

• Proven Results: Pro’Age reduces inflammation markers like CRP by 23% and oxidative stress markers like TBARS by 15%, demonstrating significant health benefits as our pets age.

• Powerful Ingredients: Utilises curcuma, cloves, rosemary, and grapefruit extract, known for their potent antioxidant and anti-inflammatory properties to fight ageing at the cellular level.

• Health Benefits: The enhanced ALLROUNDER Balancer formulation benefits all ages, but especially for senior pets to improve joint mobility, skin health, and overall vitality.

• Scientifically Backed: Pro’Age offers pet parents a proven, scientifically backed solution to enhance their pets' longevity and quality of life, giving them more happy years together.

 

Understanding ageing in pets

Ageing is a complex biological process characterised by the progressive decline in an organism’s physiological functions. As our pets get older, they’ll experience diminished immune function, musculoskeletal degeneration, cognitive impairments, and increased susceptibility to chronic diseases. Research indicates that early and preventive nutritional interventions can significantly delay the onset of age-related pathologies, promoting healthier ageing for our pets (Marrocco et al., 2017).

 

 

The role of inflammation and oxidative stress

Inflammation and oxidative stress are linked processes that play pivotal roles in ageing. The body’s natural response to injury or infection is to activate immune cells, release cytokines, and produce reactive oxygen species (ROS). While acute inflammation is essential for healing, chronic inflammation can lead to persistent tissue damage and numerous age-related diseases.

Oxidative stress arises from an imbalance between the production of ROS and the body’s antioxidant defence mechanisms. ROS, which include free radicals like superoxide anion and hydroxyl radicals, can damage cellular components such as lipids, proteins, and DNA. This damage accumulates over time and contributes to cellular dysfunction – a hallmark of the ageing process (Marrocco et al., 2017).

This interplay between oxidative stress and inflammation can lead to a never-ending cycle of cell damage and must be tightly regulated. Oxidative stress can activate pro-inflammatory pathways, while inflammation can enhance ROS production, worsening cellular damage and accelerating the ageing process (Marrocco et al., 2017).

 

Pro’Age – A natural solution for healthy ageing

Pro’Age is a scientifically formulated dietary supplement that utilises the synergistic effects of curcuma, cloves, rosemary, and grapefruit extract to limit oxidative stress and inflammation. The bioactive properties of these ingredients have been well studied and offer defence against the cellular and molecular mechanisms that underlie ageing.

Key ingredients and their mechanisms of action: 

 

• Curcuminoids (Curcuma)

Curcuminoids are polyphenolic compounds derived from turmeric with potent antioxidant and anti-inflammatory properties. They inhibit the nuclear factor-kappa B (NF-κB) pathway, reducing the transcription of pro-inflammatory cytokines. Curcuminoids also enhance the activity of endogenous antioxidant enzymes such as superoxide dismutase (SOD) and glutathione peroxidase (GPx), reducing ROS-induced damage (Hewlings & Kalman, 2017; Meizarini, 2018; Sgorlon et al., 2016).

 

• Eugenol (Cloves)

Eugenol, a phenolic compound found in cloves, has strong antioxidant activity by scavenging free radicals and chelating metal ions. It also inhibits cyclooxygenase (COX) enzymes, reducing the synthesis of pro-inflammatory prostaglandins. Eugenol’s role in the oxidative stress response contributes to its protective effects on cell structures (Adefegha et al., 2018; Bachiega et al., 2012; Barboza et al., 2018).

• Carnosic Acid (Rosemary)

Carnosic acid is a diterpene compound that protects cells from oxidative stress by activating the nuclear factor erythroid 2–related factor 2 (Nrf2) pathway. Nrf2 is a transcription factor that induces the expression of antioxidant and cytoprotective genes. Carnosic acid also inhibits the expression of pro-inflammatory cytokines, providing a dual protective effect against oxidative and inflammatory damage (Birtić et al., 2015; de Oliveira, 2016; Liu et al., 2018; Rezaie et al., 2012).

• Naringin (Grapefruit Extract)

Naringin is a flavonoid glycoside with proven antioxidant and anti-inflammatory properties. It enhances the expression of antioxidant enzymes and inhibits lipid peroxidation, protecting cellular membranes from oxidative damage. Naringin also acts on signalling pathways involved in inflammation and apoptosis, supporting cellular homeostasis and longevity (Bodas et al., 2011; Cavia-Saiz et al., 2010; Viswanatha et al., 2017).

Scientific Evidence = Proof

When it comes to our pets' health, we need more than promises—we need proof. The effectiveness of Pro’Age in the ALLROUNDER balancer is backed by scientific studies that demonstrate its significant impact on inflammation and oxidative stress.

One study involved 30 healthy dogs divided into two groups: one received a control diet, and the other received a Pro’Age-supplemented diet for four weeks. After this period, the dogs underwent a two-hour car ride to induce stress, and researchers measured the extent of the triggered inflammatory response. Figure 1 summarises the experimental design.

Figure 1: Schematic representation of the study design.

 

C-reactive protein as a marker for inflammation

A key indicator of inflammation is the body's level of C-reactive protein (CRP). High CRP levels are associated with chronic inflammation, which can lead to various health issues. Figure 2 from the Pro’Age study shows a remarkable 23% reduction in CRP levels in dogs that received Pro’Age supplementation. In contrast, the control group experienced a 12% increase in CRP levels. This difference highlights Pro’Age's powerful anti-inflammatory properties​​.

 

Figure 2: Reduction in CRP levels with Pro’Age supplementation.

 

TBARS as a marker for oxidative stress

Another critical aspect of ageing is oxidative stress, which can be measured by the levels of Thiobarbituric acid reactive substances (TBARS) in the blood. TBARS are byproducts of lipid peroxidation, a process where free radicals damage cell membranes. Figure 3 shows the effectiveness of Pro’Age in reducing TBARS levels. Dogs supplemented with Pro’Age showed a 15% decrease in TBARS, while the control group saw a 55% increase. This reduction indicates that Pro’Age significantly reduces oxidative damage, protecting cells and tissues from premature ageing​​.

 

Figure 3: Reduction in TBARS levels with Pro’Age supplementation

 

By significantly lowering both CRP and TBARS levels, Pro’Age addresses the root causes of ageing, helping your pets stay healthy, active, and happy as they age. This data can give you the confidence that the ALLROUNDER balancer, now enhanced with Pro’Age, truly delivers on its promise to support your pet's well-being!

 

Why Pro’Age matters for your pet

Okay, but what does this really mean for your pet’s health?

As your pet ages, their body’s ability to combat inflammation and oxidative stress decreases. This can lead to common age-related issues like arthritis, cognitive decline, and weakened immunity. By addressing these root causes, you can help slow down the ageing process and improve your pet’s overall quality of life.

Here are some improvements you can notice with our new ALLROUNDER balancer formulation supplemented with Pro’Age:

• Enhanced longevity and vitality

Lower oxidative stress translates to less cellular damage over time, promoting longevity and a higher quality of life. Your pet can enjoy their senior years with fewer health complications.

• Reduced joint pain and better mobility

Pro’Age can help alleviate joint pain by lowering inflammation, making it easier for your pet to move around comfortably. This is particularly beneficial for older pets who may suffer from arthritis.

• Healthier skin and coat

Chronic inflammation often leads to skin issues. By addressing the root cause, Pro’Age helps maintain healthy skin and a shiny coat, reducing the incidence of itching and irritation.

Final thoughts

We know pet owners want their furry companions to live long, healthy, and happy lives. That’s why at Bestie, we’ve taken out the guesswork for pet parents by incorporating Pro’Age into our ALLROUNDER balancer formula. This provides a natural, scientifically backed solution to help combat the effects of ageing. With regular use, you can help your pet reduce inflammation, maintain vitality, and enjoy more quality time together!

 

References

1. Adefegha, S. A., Oyeleye, S. I., Okeke, B. M., & Oboh, G. (2018). Influence of eugenol on oxidative stress biomarkers in the liver of carrageenan-induced arthritis rats. J Basic Clin Physiol Pharmacol, 30(2), 185-193. https://doi.org/10.1515/jbcpp-2018-0060
2. Bachiega, T. F., de Sousa, J. P., Bastos, J. K., & Sforcin, J. M. (2012). Clove and eugenol in noncytotoxic concentrations exert immunomodulatory/anti-inflammatory action on cytokine production by murine macrophages. J Pharm Pharmacol, 64(4), 610-616. https://doi.org/10.1111/j.2042-7158.2011.01440.x
3. Barboza, J. N., da Silva Maia Bezerra Filho, C., Silva, R. O., Medeiros, J. V. R., & de Sousa, D. P. (2018). An Overview on the Anti-inflammatory Potential and Antioxidant Profile of Eugenol. Oxid Med Cell Longev, 2018, 3957262. https://doi.org/10.1155/2018/3957262
4. Birtić, S., Dussort, P., Pierre, F. X., Bily, A. C., & Roller, M. (2015). Carnosic acid. Phytochemistry, 115, 9-19. https://doi.org/10.1016/j.phytochem.2014.12.026
5. Bodas, R., Prieto, N., López-Campos, O., Giráldez, F. J., & Andrés, S. (2011). Naringin and vitamin E influence the oxidative stability and lipid profile of plasma in lambs fed fish oil. Res Vet Sci, 91(1), 98-102. https://doi.org/10.1016/j.rvsc.2010.07.028
6. Cavia-Saiz, M., Busto, M. D., Pilar-Izquierdo, M. C., Ortega, N., Perez-Mateos, M., & Muñiz, P. (2010). Antioxidant properties, radical scavenging activity and biomolecule protection capacity of flavonoid naringenin and its glycoside naringin: a comparative study. J Sci Food Agric, 90(7), 1238-1244. https://doi.org/10.1002/jsfa.3959
7. de Oliveira, M. R. (2016). The Dietary Components Carnosic Acid and Carnosol as Neuroprotective Agents: a Mechanistic View. Mol Neurobiol, 53(9), 6155-6168. https://doi.org/10.1007/s12035-015-9519-1
8. Hewlings, S. J., & Kalman, D. S. (2017). Curcumin: A Review of Its Effects on Human Health. Foods, 6(10). https://doi.org/10.3390/foods6100092
9. Liu, M., Zhou, X., Zhou, L., Liu, Z., Yuan, J., Cheng, J., . . . Xu, J. (2018). Carnosic acid inhibits inflammation response and joint destruction on osteoclasts, fibroblast-like synoviocytes, and collagen-induced arthritis rats. J Cell Physiol, 233(8), 6291-6303. https://doi.org/10.1002/jcp.26517
10. Marrocco, I., Altieri, F., & Peluso, I. (2017). Measurement and Clinical Significance of Biomarkers of Oxidative Stress in Humans. Oxid Med Cell Longev, 2017, 6501046. https://doi.org/10.1155/2017/6501046
11. Meizarini, A. S., Siswandono & Riawan, Wibi & Rahayu, Retno. (2018). n silico and in vivo anti-inflammatory studies of curcuminoids, turmeric extract with Zinc oxide, and eugenol. In (Vol. 17, pp. 269): Tropical Journal of Pharmaceutical Research.
12. Rezaie, T., McKercher, S. R., Kosaka, K., Seki, M., Wheeler, L., Viswanath, V., . . . Lipton, S. A. (2012). Protective effect of carnosic acid, a pro-electrophilic compound, in models of oxidative stress and light-induced retinal degeneration. Invest Ophthalmol Vis Sci, 53(12), 7847-7854. https://doi.org/10.1167/iovs.12-10793
13. Sgorlon, S., Stefanon, B., Sandri, M., & Colitti, M. (2016). Nutrigenomic activity of plant derived compounds in health and disease: Results of a dietary intervention study in dog. Res Vet Sci, 109, 142-148. https://doi.org/10.1016/j.rvsc.2016.10.005
14. Viswanatha, G. L., Shylaja, H., & Moolemath, Y. (2017). The beneficial role of Naringin- a citrus bioflavonoid, against oxidative stress-induced neurobehavioral disorders and cognitive dysfunction in rodents: A systematic review and meta-analysis. Biomed Pharmacother, 94, 909-929. https://doi.org/10.1016/j.biopha.2017.07.072